Since our experiments are highly dependent on when spawns of larvae are ready, once they were finally developed, we visited Martha’s Vineyard shellfish group to pick them up. I’ve never been to Martha’s Vineyard (MV for short) and was extremely excited at the prospect of getting on the ferry, especially since I hear MV was simply a more touristy version of Woods Hole and wouldn’t have went out of my way to visit there otherwise.
After getting the larvae, which were collected on 200um filters so we have a lower size limit, we check for their competency to settle by looking for eyespots (which develop in late-stage larvae).
Since these larvae are already competent to settle, our experiments are extremely time-sensitive. We have to transfer them into bleached culture buckets, set up the airstone system, feed them, and start the experiment as soon as possible. We typically have 2-3 days after larval acquisition to perform the experiments, and multiple people in the lab take shifts to realize the continuous stint of data collection in these 2-3 days.
I have to admit that it was a tiring three days, but Lauren, our advisor, was super supportive, took quite a few shifts herself, and was kind enough to bring us food and munchkins (did you know that this was the “Timbits” of the US? so cute!). Additionally, after some level of sleep deprivation, random things become hilarious, so we had a pretty great time derping around the lab while waiting for the data to finish downloading after each collection (each dataset contains several gigabytes of images). Overall, the experiments went very well and we’ve successfully collected enough data to work on through the next few months.